Introduction
Before entrusting your valuable peptide samples to a testing laboratory, it's essential to ask the right questions. This guide covers the five most critical questions that will help you evaluate potential laboratory partners.
Question 1: What Accreditations Do You Hold?
This should be your first question. Accreditation demonstrates that a laboratory meets internationally recognized standards for technical competence.
What to look for:
- documented method validation for specific test methods
- GLP certification if data will support regulatory submissions
- CLIA certification for clinical applications
Follow-up questions:
- Can you provide copies of your accreditation certificates?
- What is the scope of your accreditation?
- When was your last audit?
Question 2: What Methods Do You Use for Peptide Analysis?
Understanding the analytical methods ensures they meet your specific requirements.
Key methods to inquire about:
- HPLC purity analysis (RP-HPLC, SEC-HPLC)
- Mass spectrometry for identity confirmation
- Amino acid analysis for composition
- Endotoxin and bioburden testing
Follow-up questions:
- Are your methods validated?
- Can you develop custom methods for unique peptides?
- What detection limits can you achieve?
Question 3: What Is Your Sample Handling Protocol?
Proper sample handling is crucial for accurate results, especially for sensitive peptides.
Important considerations:
- Temperature-controlled storage and shipping
- Chain of custody documentation
- Minimum sample requirements
- Sample retention policies
