Cyanocobalamin (Vitamin B12) Testing Services
Cyanocobalamin is the most common pharmaceutical form of vitamin B12. It is used in injectable B12 preparations and frequently appears in compounded blends. Identity, purity, and concentration verification are critical for compounding pharmacies.
Research Applications
Common research areas for Cyanocobalamin (Vitamin B12)
- B12 deficiency research
- Compounded injectable QC
- Methylation pathway studies
- Hematology research
Peptide Information
Molecular details and sample requirements
Sequence / Structure:
Cobalamin (small molecule)
Molecular Weight
1355.37 g/mol
Formula
C63H88CoN14O14P
Recommended Purity
≥95%
Sample Size
1-2 mg lyophilized
Our Cyanocobalamin (Vitamin B12) Testing Services
Comprehensive analytical testing to ensure Cyanocobalamin (Vitamin B12) quality and purity
Purity Testing
HPLC analysis to verify Cyanocobalamin (Vitamin B12) purity levels
Identity Verification
Mass spectrometry to confirm molecular identity
Third-Party Verification
Independent COA verification for Cyanocobalamin (Vitamin B12)
Certificate of Analysis
Official US laboratory documentation
Ready to test your peptides?
Send your peptide name, sample count, and the tests you need. We return a fixed-price quote, usually within one business day.
- validated analytical methods, independent of any supplier
- 9-11 business day standard turnaround
- Traceable COA with chromatograms and method parameters
Cyanocobalamin (Vitamin B12) Testing Methodology
Our analytical workflow for Cyanocobalamin (Vitamin B12) follows USP <1225> guidance for validated chromatographic methods. Each lot is processed by a credentialed analyst with documented chain of custody.
Step 1
Sample intake & chain of custody
Your Cyanocobalamin (Vitamin B12) sample (1-2 mg lyophilized) is logged at our Florida lab with a unique ID, photographed, and stored at -20 °C until analysis.
Step 2
Reverse-phase HPLC purity
Cyanocobalamin (Vitamin B12) is dissolved in mobile phase and run on a C18 column with a water/acetonitrile gradient containing 0.1% TFA. UV detection at 214 nm quantifies the main peak and any related impurities.
Step 3
LC-MS identity confirmation
An aliquot is analyzed by liquid chromatography-mass spectrometry. The observed monoisotopic mass is compared against the theoretical molecular weight (1355.37 g/mol) for Cyanocobalamin (Vitamin B12) to confirm identity.
Step 4
Impurity profiling & reporting
Related substances, truncations, and oxidation products are quantified. Findings are compiled into a signed Certificate of Analysis with chromatograms, mass spectra, and analyst interpretation.
Cyanocobalamin (Vitamin B12) Purity Notes
Recommended research-grade purity for Cyanocobalamin (Vitamin B12) is ≥95%. Lot-to-lot variation is common with solid-phase synthesis, so independent verification matters.
Common impurities to watch
- Truncated sequences from incomplete coupling
- Deletion peptides missing one or more residues
- Oxidation of methionine, tryptophan, or cysteine
- Residual TFA, acetate, or solvent counter-ions
- Aggregates and dimers from improper storage
What our COA confirms
- Main peak purity by HPLC area %
- Observed vs theoretical mass (1355.37 g/mol)
- Named impurities ≥ 0.1% area
- Net peptide content where applicable
- Method, column, and instrument metadata
Cyanocobalamin (Vitamin B12) Handling, Storage & Safety
Guidance below is for laboratory and research personnel only. Cyanocobalamin (Vitamin B12) is supplied for research use and is not intended for human or veterinary use.
Storage
Store lyophilized Cyanocobalamin (Vitamin B12) at -20 °C, protected from light and moisture. After reconstitution, aliquot and store at -80 °C; avoid repeated freeze-thaw cycles.
Reconstitution
Reconstitute in bacteriostatic or sterile water in a clean environment. Allow vials to reach room temperature before opening to prevent moisture condensation on the powder.
PPE & shipping
Handle with gloves, lab coat, and eye protection. Ship samples to ACS overnight on dry ice or cold packs in a rigid, leak-proof container with a packing slip referencing your quote.
This page is informational and does not constitute medical advice. Consult your institution's safety officer and SDS before working with Cyanocobalamin (Vitamin B12).
Related Peptides
Hexarelin Testing
Hexarelin is a synthetic hexapeptide and one of the most potent growth hormone s...
AOD-9604 Testing
AOD-9604 is a modified fragment of human growth hormone (hGH), specifically the ...
Retatrutide Testing
Retatrutide (LY3437943) is a novel triple agonist peptide targeting GIP, GLP-1, ...