LC-MS/MS
Liquid Chromatography combined with tandem Mass Spectrometry for both purity and identity in one run.
Definition
Liquid Chromatography–Tandem Mass Spectrometry, combines HPLC separation with two stages of mass analysis. Provides both purity data and structural information, enabling sequence confirmation and impurity characterization.
In More Detail
LC-MS/MS first separates components by HPLC, then performs two rounds of mass analysis. The first stage isolates a precursor ion, which is fragmented in a collision cell. The second stage measures the resulting fragment ions. The fragment pattern is characteristic of the peptide sequence and is used to confirm amino acid order and pinpoint the location of modifications or impurities.