Humanin Testing Services
Humanin is a 24-amino acid mitochondrial-derived peptide encoded within the 16S rRNA gene of mitochondrial DNA. It is studied for its cytoprotective, neuroprotective, and metabolic effects, and its decline with age makes it a focus of longevity research.
Research Applications
Common research areas for Humanin
- Neuroprotection research
- Mitochondrial biology
- Aging studies
- Metabolic research
Peptide Information
Molecular details and sample requirements
Sequence / Structure:
Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala
Molecular Weight
2687.27 g/mol
Formula
C115H191N37O30S2
Recommended Purity
≥95%
Sample Size
1-2 mg lyophilized
Our Humanin Testing Services
Comprehensive analytical testing to ensure Humanin quality and purity
Purity Testing
HPLC analysis to verify Humanin purity levels
Identity Verification
Mass spectrometry to confirm molecular identity
Third-Party Verification
Independent COA verification for Humanin
Certificate of Analysis
Official US laboratory documentation
Ready to test your peptides?
Send your peptide name, sample count, and the tests you need. We return a fixed-price quote, usually within one business day.
- validated analytical methods, independent of any supplier
- 9-11 business day standard turnaround
- Traceable COA with chromatograms and method parameters
Humanin Testing Methodology
Our analytical workflow for Humanin follows USP <1225> guidance for validated chromatographic methods. Each lot is processed by a credentialed analyst with documented chain of custody.
Step 1
Sample intake & chain of custody
Your Humanin sample (1-2 mg lyophilized) is logged at our Florida lab with a unique ID, photographed, and stored at -20 °C until analysis.
Step 2
Reverse-phase HPLC purity
Humanin is dissolved in mobile phase and run on a C18 column with a water/acetonitrile gradient containing 0.1% TFA. UV detection at 214 nm quantifies the main peak and any related impurities.
Step 3
LC-MS identity confirmation
An aliquot is analyzed by liquid chromatography-mass spectrometry. The observed monoisotopic mass is compared against the theoretical molecular weight (2687.27 g/mol) for Humanin to confirm identity.
Step 4
Impurity profiling & reporting
Related substances, truncations, and oxidation products are quantified. Findings are compiled into a signed Certificate of Analysis with chromatograms, mass spectra, and analyst interpretation.
Humanin Purity Notes
Recommended research-grade purity for Humanin is ≥95%. Lot-to-lot variation is common with solid-phase synthesis, so independent verification matters.
Common impurities to watch
- Truncated sequences from incomplete coupling
- Deletion peptides missing one or more residues
- Oxidation of methionine, tryptophan, or cysteine
- Residual TFA, acetate, or solvent counter-ions
- Aggregates and dimers from improper storage
What our COA confirms
- Main peak purity by HPLC area %
- Observed vs theoretical mass (2687.27 g/mol)
- Named impurities ≥ 0.1% area
- Net peptide content where applicable
- Method, column, and instrument metadata
Humanin Handling, Storage & Safety
Guidance below is for laboratory and research personnel only. Humanin is supplied for research use and is not intended for human or veterinary use.
Storage
Store lyophilized Humanin at -20 °C, protected from light and moisture. After reconstitution, aliquot and store at -80 °C; avoid repeated freeze-thaw cycles.
Reconstitution
Reconstitute in bacteriostatic or sterile water in a clean environment. Allow vials to reach room temperature before opening to prevent moisture condensation on the powder.
PPE & shipping
Handle with gloves, lab coat, and eye protection. Ship samples to ACS overnight on dry ice or cold packs in a rigid, leak-proof container with a packing slip referencing your quote.
This page is informational and does not constitute medical advice. Consult your institution's safety officer and SDS before working with Humanin.
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