Methylcobalamin (Methyl-B12) Testing Services
Methylcobalamin is the active, methylated form of vitamin B12 used in methylation pathways. It is common in compounded injectables and benefits from independent identity and concentration testing.
Research Applications
Common research areas for Methylcobalamin (Methyl-B12)
- Methylation research
- B12 deficiency studies
- Compounded injectable QC
- Neurological B12 research
Peptide Information
Molecular details and sample requirements
Sequence / Structure:
Methylated cobalamin (small molecule)
Molecular Weight
1344.38 g/mol
Formula
C63H91CoN13O14P
Recommended Purity
≥95%
Sample Size
1-2 mg lyophilized
Our Methylcobalamin (Methyl-B12) Testing Services
Comprehensive analytical testing to ensure Methylcobalamin (Methyl-B12) quality and purity
Purity Testing
HPLC analysis to verify Methylcobalamin (Methyl-B12) purity levels
Identity Verification
Mass spectrometry to confirm molecular identity
Third-Party Verification
Independent COA verification for Methylcobalamin (Methyl-B12)
Certificate of Analysis
Official US laboratory documentation
Ready to test your peptides?
Send your peptide name, sample count, and the tests you need. We return a fixed-price quote, usually within one business day.
- validated analytical methods, independent of any supplier
- 9-11 business day standard turnaround
- Traceable COA with chromatograms and method parameters
Methylcobalamin (Methyl-B12) Testing Methodology
Our analytical workflow for Methylcobalamin (Methyl-B12) follows USP <1225> guidance for validated chromatographic methods. Each lot is processed by a credentialed analyst with documented chain of custody.
Step 1
Sample intake & chain of custody
Your Methylcobalamin (Methyl-B12) sample (1-2 mg lyophilized) is logged at our Florida lab with a unique ID, photographed, and stored at -20 °C until analysis.
Step 2
Reverse-phase HPLC purity
Methylcobalamin (Methyl-B12) is dissolved in mobile phase and run on a C18 column with a water/acetonitrile gradient containing 0.1% TFA. UV detection at 214 nm quantifies the main peak and any related impurities.
Step 3
LC-MS identity confirmation
An aliquot is analyzed by liquid chromatography-mass spectrometry. The observed monoisotopic mass is compared against the theoretical molecular weight (1344.38 g/mol) for Methylcobalamin (Methyl-B12) to confirm identity.
Step 4
Impurity profiling & reporting
Related substances, truncations, and oxidation products are quantified. Findings are compiled into a signed Certificate of Analysis with chromatograms, mass spectra, and analyst interpretation.
Methylcobalamin (Methyl-B12) Purity Notes
Recommended research-grade purity for Methylcobalamin (Methyl-B12) is ≥95%. Lot-to-lot variation is common with solid-phase synthesis, so independent verification matters.
Common impurities to watch
- Truncated sequences from incomplete coupling
- Deletion peptides missing one or more residues
- Oxidation of methionine, tryptophan, or cysteine
- Residual TFA, acetate, or solvent counter-ions
- Aggregates and dimers from improper storage
What our COA confirms
- Main peak purity by HPLC area %
- Observed vs theoretical mass (1344.38 g/mol)
- Named impurities ≥ 0.1% area
- Net peptide content where applicable
- Method, column, and instrument metadata
Methylcobalamin (Methyl-B12) Handling, Storage & Safety
Guidance below is for laboratory and research personnel only. Methylcobalamin (Methyl-B12) is supplied for research use and is not intended for human or veterinary use.
Storage
Store lyophilized Methylcobalamin (Methyl-B12) at -20 °C, protected from light and moisture. After reconstitution, aliquot and store at -80 °C; avoid repeated freeze-thaw cycles.
Reconstitution
Reconstitute in bacteriostatic or sterile water in a clean environment. Allow vials to reach room temperature before opening to prevent moisture condensation on the powder.
PPE & shipping
Handle with gloves, lab coat, and eye protection. Ship samples to ACS overnight on dry ice or cold packs in a rigid, leak-proof container with a packing slip referencing your quote.
This page is informational and does not constitute medical advice. Consult your institution's safety officer and SDS before working with Methylcobalamin (Methyl-B12).
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