Skip to main content
Skip to main content

HPLC Purity Testing Cost Per Sample

Independent third-party HPLC purity testing at ACS starts at $150 per sample. Full contaminant panels for injectable-grade material run $500 to $1,200. Here is the complete breakdown so you can budget accurately and pick the right panel.

Get an Exact Quote

Purity only

$150–$300 per sample. HPLC purity + integration + signed COA with chromatogram.

Purity + identity

$250–$450 per sample. Adds LC-MS mass confirmation to purity.

Full injectable panel

$500–$1,200 per sample. Purity, identity, endotoxin, heavy metals, residual solvents.

Standard turnaround

5–7 business days from sample receipt for all panels.

What each panel includes

PanelIncludesPrice rangeTypical use
HPLC purityHPLC + area % + chromatogram$150–$300Baseline purity verification
Purity + identityHPLC + LC-MS confirmation$250–$450Confirms molecule + purity
Endotoxin (LAL)Kinetic chromogenic USP <85>$150–$300Any injectable
Heavy metals (ICP-MS)USP <232>/<233>$200–$400Pharmacy 503A/503B
Residual solventsGC-headspace USP <467>$200–$400Injectable-grade release
Full injectable panelAll of the above bundled$500–$1,200Compounding pharmacy release

Volume discounts

Per-sample pricing drops at defined thresholds:

  • 1–4 samples: standard pricing
  • 5–19 samples: approximately 10–15% reduction
  • 20+ samples: contract pricing available
  • Standing quarterly programs: bespoke pricing

Why prices vary between labs

Meaningful comparison is on methodology, not sticker price. A $75 purity test that skips method validation, uses a generic gradient, and returns a number without a chromatogram is not the same product as a $250 test that includes validated method conditions, integrated impurity profile, and raw data release. Look for documented method validation and USP compendial method compliance before comparing prices.

See also: in-house vs third-party testing, how to read a peptide COA, and the purity testing service overview.

Purity Testing Cost: FAQ

HPLC purity testing typically costs $150 to $300 per sample at an accredited third-party lab. Higher-resolution methods, larger sample sets, and expedited handling can shift the number. Bundled contaminant panels (endotoxin, heavy metals, residual solvents) run $500 to $1,200 per sample.

Sample volume, sequence complexity, method requirements, and reporting depth all affect the price. A single HPLC run on a common peptide with a validated method is the low end. A custom method development for a novel modified peptide with LC-MS confirmation is the high end.

Yes. Per-sample pricing drops meaningfully at 5+ samples and again at 20+. Compounding pharmacies and research labs running quarterly QA panels typically qualify for standing customer pricing.

A standard purity test includes sample prep, HPLC analysis, integration, and a signed certificate of analysis with the chromatogram attached. Raw data files are available on request at no extra charge.

For anything intended for injection, yes. A panel adds endotoxin (LAL), heavy metals (ICP-MS), and residual solvents (GC) to purity and identity. Together they answer the 'is this safe to inject' question that purity alone cannot.

The manufacturer COA is bundled into the peptide price. Third-party testing is billed separately. The comparison is not price for price; it is whether independent verification is worth $150 to $1,200 on a sample that could cost hundreds to thousands of dollars if it fails downstream.

Standard turnaround is 5 to 7 business days from sample receipt. Expedited handling is not offered because rushing analytical work is where errors are introduced.

Submit peptide name, sequence if available, sample count, and which tests you need at acslabtest.com/get-quote. Quotes are typically returned within one business day.

Ready to Get Started?

Contact us today for a free consultation and customized quote for your peptide testing needs.

(561) 513-8435cpierre@acslabtest.com