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HPLC Purity Testing Cost Per Sample

Independent third-party HPLC purity testing at ACS starts at $150 per sample. Full contaminant panels for injectable-grade material run $500 to $1,200. Here is the complete breakdown so you can budget accurately and pick the right panel.

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Purity only

$150–$300 per sample. HPLC purity + integration + signed COA with chromatogram.

Purity + identity

$250–$450 per sample. Adds LC-MS mass confirmation to purity.

Full injectable panel

$500–$1,200 per sample. Purity, identity, endotoxin, heavy metals, residual solvents.

Standard turnaround

9–11 business days from sample receipt for all panels.

What each panel includes

PanelIncludesPrice rangeTypical use
HPLC purityHPLC + area % + chromatogram$150–$300Baseline purity verification
Purity + identityHPLC + LC-MS confirmation$250–$450Confirms molecule + purity
Endotoxin (LAL)Kinetic chromogenic USP <85>$150–$300Any injectable
Heavy metals (ICP-MS)USP <232>/<233>$200–$400Pharmacy 503A/503B
Residual solventsGC-headspace USP <467>$200–$400Injectable-grade release
Full injectable panelAll of the above bundled$500–$1,200Compounding pharmacy release

Volume discounts

Per-sample pricing drops at defined thresholds:

  • 1–4 samples: standard pricing
  • 5–19 samples: approximately 10–15% reduction
  • 20+ samples: contract pricing available
  • Standing quarterly programs: bespoke pricing

Why prices vary between labs

Meaningful comparison is on methodology, not sticker price. A $75 purity test that skips method validation, uses a generic gradient, and returns a number without a chromatogram is not the same product as a $250 test that includes validated method conditions, integrated impurity profile, and raw data release. Look for documented method validation and USP compendial method compliance before comparing prices.

See also: in-house vs third-party testing, how to read a peptide COA, and the purity testing service overview.

Purity Testing Cost: FAQ

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